Show simple item record

dc.contributor.authorRiboulet-Bisson, Eliette*
dc.contributor.authorSturme, Mark H. J.*
dc.contributor.authorJeffery, Ian B*
dc.contributor.authorO'Donnell, Michelle M.*
dc.contributor.authorNeville, B. Anne*
dc.contributor.authorForde, Brian M*
dc.contributor.authorClaesson, Marcus J.*
dc.contributor.authorHarris, Hugh*
dc.contributor.authorGardiner, Gillian E.*
dc.contributor.authorCasey, Patrick G.*
dc.contributor.authorLawlor, Peadar G*
dc.contributor.authorO'Toole, Paul W.*
dc.contributor.authorRoss, R Paul*
dc.date.accessioned2014-09-03T11:35:15Z
dc.date.available2014-09-03T11:35:15Z
dc.date.issued17/02/2012
dc.identifier.citationRiboulet-Bisson E, Sturme MHJ, Jeffery IB, O'Donnell MM, Neville BA, et al. (2012) Effect of Lactobacillus salivarius Bacteriocin Abp118 on the Mouse and Pig Intestinal Microbiota. PLoS ONE 7(2): e31113. doi:10.1371/journal.pone.0031113en_GB
dc.identifier.urihttp://hdl.handle.net/11019/720
dc.descriptionpeer-revieweden_GB
dc.description.abstractLactobacilli are Gram-positive bacteria that are a subdominant element in the human gastrointestinal microbiota, and which are commonly used in the food industry. Some lactobacilli are considered probiotic, and have been associated with health benefits. However, there is very little culture-independent information on how consumed probiotic microorganisms might affect the entire intestinal microbiota. We therefore studied the impact of the administration of Lactobacillus salivarius UCC118, a microorganism well characterized for its probiotic properties, on the composition of the intestinal microbiota in two model animals. UCC118 has anti-infective activity due to production of the bacteriocin Abp118, a broad-spectrum class IIb bacteriocin, which we hypothesized could impact the microbiota. Mice and pigs were administered wild-type (WT) L. salivarius UCC118 cells, or a mutant lacking bacteriocin production. The microbiota composition was determined by pyrosequencing of 16S rRNA gene amplicons from faeces. The data show that L. salivarius UCC118 administration had no significant effect on proportions of major phyla comprising the mouse microbiota, whether the strain was producing bacteriocin or not. However, L. salivarius UCC118 WT administration led to a significant decrease in Spirochaetes levels, the third major phylum in the untreated pig microbiota. In both pigs and mice, L. salivarius UCC118 administration had an effect on Firmicutes genus members. This effect was not observed when the mutant strain was administered, and was thus associated with bacteriocin production. Surprisingly, in both models, L. salivarius UCC118 administration and production of Abp118 had an effect on Gram-negative microorganisms, even though Abp118 is normally not active in vitro against this group of microorganisms. Thus L. salivarius UCC118 administration has a significant but subtle impact on mouse and pig microbiota, by a mechanism that seems at least partially bacteriocin-dependenten_GB
dc.description.sponsorshipThis work was supported by a Principal Investigator award (07/IN.1/B1780) from Science Foundation Ireland to Dr. O'Toole. Dr. Neville was the recipient of an Embark studentship from the Irish Research Council for Science Engineering and Technology.
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLOS ONE;vol 7
dc.subjectLactobacillus salivariusen_GB
dc.subjectBacterial pathogensen_GB
dc.subjectIntestinal microbiotaen_GB
dc.subjectProbioticen_GB
dc.subjectMouseen_GB
dc.subjectPigen_GB
dc.titleEffect of Lactobacillus salivarius Bacteriocin Abp118 on the Mouse and Pig Intestinal Microbiotaen_GB
dc.typeArticleen_GB
dc.identifier.rmisPDPG-0231-5822
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0031113
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorIrish Research Council for Science, Engineering and Technology
dc.contributor.sponsorGrantNumber07/IN.1/B1780
refterms.dateFOA2018-01-12T08:10:01Z


Files in this item

Thumbnail
Name:
PLoSOne e31113.pdf
Size:
906.4Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record