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dc.contributor.authorNeville, B. Anne
dc.contributor.authorForde, Brian M.
dc.contributor.authorClaesson, Marcus J.
dc.contributor.authorDarby, Trevor
dc.contributor.authorCoghlan, Avril
dc.contributor.authorNally, Kenneth
dc.contributor.authorRoss, R Paul
dc.contributor.authorO'Toole, Paul W.
dc.date.accessioned2014-09-08T15:55:43Z
dc.date.available2014-09-08T15:55:43Z
dc.date.issued10/07/2012
dc.identifier.citationNeville BA, Forde BM, Claesson MJ, Darby T, Coghlan A, et al. (2012) Characterization of Pro-Inflammatory Flagellin Proteins Produced by Lactobacillus ruminis and Related Motile Lactobacilli. PLoS ONE 7(7): e40592. doi:10.1371/journal.pone.0040592en_GB
dc.identifier.urihttp://hdl.handle.net/11019/726
dc.descriptionpeer-revieweden_GB
dc.description.abstractLactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus Lactobacillus. Of these, only L. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cows. Nine L. ruminis strains were investigated here to elucidate the biochemistry and genetics of Lactobacillus motility. Six strains isolated from humans were non-motile while three bovine isolates were motile. A complete set of flagellum biogenesis genes was annotated in the sequenced genomes of two strains, ATCC25644 (human isolate) and ATCC27782 (bovine isolate), but only the latter strain produced flagella. Comparison of the L. ruminis and L. mali DSM20444T motility loci showed that their genetic content and gene-order were broadly similar, although the L. mali motility locus was interrupted by an 11.8 Kb region encoding rhamnose utilization genes that is absent from the L. ruminis motility locus. Phylogenetic analysis of 39 motile bacteria indicated that Lactobacillus motility genes were most closely related to those of motile carnobacteria and enterococci. Transcriptome analysis revealed that motility genes were transcribed at a significantly higher level in motile L. ruminis ATCC27782 than in non-motile ATCC25644. Flagellin proteins were isolated from L. ruminis ATCC27782 and from three other Lactobacillus species, while recombinant flagellin of aflagellate L. ruminis ATCC25644 was expressed and purified from E. coli. These native and recombinant Lactobacillus flagellins, and also flagellate L. ruminis cells, triggered interleukin-8 production in cultured human intestinal epithelial cells in a manner suppressed by short interfering RNA directed against Toll-Like Receptor 5. This study provides genetic, transcriptomic, phylogenetic and immunological insights into the trait of flagellum-mediated motility in the lactobacilli.en_GB
dc.description.sponsorshipThis work was supported by a Principal Investigator Award (07/IN.1/B1780) from Science Foundation Ireland to PWOT. BAN was the recipient of an Embark studentship from the Irish Research Council for Science Engineering and Technology. TD and KN were supported by the Alimentary Pharmabiotic Centre, funded by Science Foundation Ireland.
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLOS ONE;vol 7
dc.subjectLactobacillus ruminisen_GB
dc.subjectGastrointestinal microbiotaen_GB
dc.subjectFlagellar motilityen_GB
dc.subjectFlagellinen_GB
dc.subjectPhylogenetic analysisen_GB
dc.subjectGenetic locien_GB
dc.subjectTranscriptome analysisen_GB
dc.titleCharacterization of Pro-Inflammatory Flagellin Proteins Produced by Lactobacillus ruminis and Related Motile Lactobacillien_GB
dc.typeArticleen_GB
dc.identifier.rmisMDBY-0106-5972
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0040592
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorIrish Research Council for Science, Engineering and Technology
dc.contributor.sponsorGrantNumber07/IN.1/B1780
refterms.dateFOA2018-01-12T08:10:59Z


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