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dc.contributor.authorWhelehan, Cormac J*
dc.contributor.authorBarry-Reidy, Anne*
dc.contributor.authorMeade, Kieran G*
dc.contributor.authorEckersall, P David*
dc.contributor.authorChapwanya, Aspinas*
dc.contributor.authorNarciandi, Fernando*
dc.contributor.authorLloyd, Andrew T*
dc.contributor.authorO'Farrelly, Cliona*
dc.date.accessioned2014-10-10T15:48:27Z
dc.date.available2014-10-10T15:48:27Z
dc.date.issued2014-02-13
dc.identifier.citationWhelehan et al. Characterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissue. BMC Genomics, 2014, 15:128. doi:10.1186/1471-2164-15-128en_GB
dc.identifier.urihttp://hdl.handle.net/11019/729
dc.descriptionpeer-revieweden_GB
dc.descriptionThis work was supported by an Irish Department of Agriculture, Fisheries and Food Research Stimulus Fund Grant (RSF 06-340).
dc.description.abstractAbstract Background Cathelicidins comprise a major group of host-defence peptides. Conserved across a wide range of species, they have several functions related to host defence. Only one cathelicidin has been found in humans but several cathelicidin genes occur in the bovine genome. We propose that these molecules may have a protective role against mastitis. The aim of this study was to characterise the cathelicidin gene-cluster in the bovine genome and to identify sites of expression in the bovine mammary gland. Results Bioinformatic analysis of the bovine genome (BosTau7) revealed seven protein-coding cathelicidin genes, CATHL1-7, including two identical copies of CATHL4, as well as three additional putative cathelicidin genes, all clustered on the long arm of chromosome 22. Six of the seven protein-coding genes were expressed in leukocytes extracted from milk of high somatic cell count (SCC) cows. CATHL5 was expressed across several sites in the mammary gland, but did not increase in response to Staphylococcus aureus infection. Conclusions Here, we characterise the bovine cathelicidin gene cluster and reconcile inconsistencies in the datasets of previous studies. Constitutive cathelicidin expression in the mammary gland suggests a possible role for these host defence peptides its protection. Background Cathelicidins comprise a major group of host-defence peptides. Conserved across a wide range of species, they have several functions related to host defence. Only one cathelicidin has been found in humans but several cathelicidin genes occur in the bovine genome. We propose that these molecules may have a protective role against mastitis. The aim of this study was to characterise the cathelicidin gene-cluster in the bovine genome and to identify sites of expression in the bovine mammary gland. Results Bioinformatic analysis of the bovine genome (BosTau7) revealed seven protein-coding cathelicidin genes, CATHL1-7, including two identical copies of CATHL4, as well as three additional putative cathelicidin genes, all clustered on the long arm of chromosome 22. Six of the seven protein-coding genes were expressed in leukocytes extracted from milk of high somatic cell count (SCC) cows. CATHL5 was expressed across several sites in the mammary gland, but did not increase in response to Staphylococcus aureus infection. Conclusions Here, we characterise the bovine cathelicidin gene cluster and reconcile inconsistencies in the datasets of previous studies. Constitutive cathelicidin expression in the mammary gland suggests a possible role for these host defence peptides its protection.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Genomics;vol 15
dc.subjectCathelicidinen_GB
dc.subjectHidden Markov Model (HMM),en_GB
dc.subjectGene clusteren_GB
dc.subjectLocusen_GB
dc.subjectTissue expressionen_GB
dc.titleCharacterisation and expression profile of the bovine cathelicidin gene repertoire in mammary tissueen_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2164-15-128
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
refterms.dateFOA2018-04-20T15:30:17Z


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