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dc.contributor.authorFatima, Attia*
dc.contributor.authorLynn, David J*
dc.contributor.authorO'Boyle, Padraic*
dc.contributor.authorSeoighe, Cathal*
dc.contributor.authorMorris, Dermot G.*
dc.date.accessioned2014-10-20T15:20:09Z
dc.date.available2014-10-20T15:20:09Z
dc.date.issued2014-04-12
dc.identifier.citationAttia Fatima, et al. The miRNAome of the postpartum dairy cow liver in negative energy balance. BMC Genomics, 2014, 15:279. DOI: 10.1186/1471-2164-15-279en_GB
dc.identifier.urihttp://hdl.handle.net/11019/732
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground: Negative energy balance (NEB) is an altered metabolic state in high yielding cows that occurs during the first few weeks postpartum when energy demands for lactation and maintenance exceed the energy supply from dietary intake. NEB can, in turn, lead to metabolic disorders and to reduced fertility. Alterations in the expression of more than 700 hepatic genes have previously been reported in a study of NEB in postpartum dairy cows. miRNAs (microRNA) are known to mediate many alterations in gene expression post transcriptionally. To study the hepatic miRNA content of postpartum dairy cows, including their overall abundance and differential expression, in mild NEB (MNEB) and severe NEB (SNEB), short read RNA sequencing was carried out. To identify putative targets of differentially expressed miRNAs among differentially expressed hepatic genes reported previously in dairy cows in SNEB computational target identification was employed. Results: Our results indicate that the dairy cow liver expresses 53 miRNAs at a lower threshold of 10 reads per million. Of these, 10 miRNAs accounted for greater than 95% of the miRNAome (miRNA content). Of the highly expressed miRNAs, miR-122 constitutes 75% followed by miR-192 and miR-3596. Five out of thirteen let-7 miRNA family members are also among the highly expressed miRNAs. miR-143, down-regulated in SNEB, was found to have 4 putative up-regulated gene targets associated with SNEB including LRP2 (low density lipoprotein receptor-related protein 2), involved in lipid metabolism and up-regulated in SNEB. Conclusions: This is the first liver miRNA-seq profiling study of moderate yielding dairy cows in the early postpartum period. Tissue specific miR-122 and liver enriched miR-192 are two of the most abundant miRNAs in the postpartum dairy cow liver. miR-143 is significantly down-regulated in SNEB and putative targets of miRNA-143 which are up-regulated in SNEB, include a gene involved in lipid metabolism.en_GB
dc.description.sponsorshipTeagasc Walsh Fellowship Programmeen_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Genomics;vol 15
dc.subjectMicro-RNAen_GB
dc.subjectRNA-seqen_GB
dc.subjectLiveren_GB
dc.subjectNegative energy balanceen_GB
dc.subjectNutritionen_GB
dc.subjectDairy Cattleen_GB
dc.titleThe miRNAome of the postpartum dairy cow liver in negative energy balanceen_GB
dc.typeArticleen_GB
dc.identifier.rmisGEAB-0201-6033
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2164-15-279
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
refterms.dateFOA2018-01-12T08:11:01Z


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