Show simple item record

dc.contributor.authorField, Des*
dc.contributor.authorBegley, Maire*
dc.contributor.authorO'Connor, Paula M.*
dc.contributor.authorDaly, Karen M.*
dc.contributor.authorHugenholtz, Floor*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorHill, Colin*
dc.contributor.authorRoss, R Paul*
dc.date.accessioned2015-01-30T12:41:51Z
dc.date.available2015-01-30T12:41:51Z
dc.date.issued08/10/2012
dc.identifier.citationField D, Begley M, O’Connor PM, Daly KM, Hugenholtz F, et al. (2012) Bioengineered Nisin A Derivatives with Enhanced Activity against Both Gram Positive and Gram Negative Pathogens. PLoS ONE 7(10): e46884. doi:10.1371/journal.pone.0046884en_GB
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11019/769
dc.descriptionpeer-revieweden_GB
dc.description.abstractNisin is a bacteriocin widely utilized in more than 50 countries as a safe and natural antibacterial food preservative. It is the most extensively studied bacteriocin, having undergone decades of bioengineering with a view to improving function and physicochemical properties. The discovery of novel nisin variants with enhanced activity against clinical and foodborne pathogens has recently been described. We screened a randomized bank of nisin A producers and identified a variant with a serine to glycine change at position 29 (S29G), with enhanced efficacy against S. aureus SA113. Using a site-saturation mutagenesis approach we generated three more derivatives (S29A, S29D and S29E) with enhanced activity against a range of Gram positive drug resistant clinical, veterinary and food pathogens. In addition, a number of the nisin S29 derivatives displayed superior antimicrobial activity to nisin A when assessed against a range of Gram negative food-associated pathogens, including E. coli, Salmonella enterica serovar Typhimurium and Cronobacter sakazakii. This is the first report of derivatives of nisin, or indeed any lantibiotic, with enhanced antimicrobial activity against both Gram positive and Gram negative bacteria.en_GB
dc.description.sponsorshipThis work was supported by the Irish Government under the National Development Plan, through Science Foundation Ireland Investigator awards (10/IN.1/B3027) and (06/IN.1/B98) (http://www.sfi.ie).en_GB
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLOS ONE;vol 7
dc.subjectNisin A derivativesen_GB
dc.subjectGram negative bacteriaen_GB
dc.subjectEnhanced antimicrobial activityen_GB
dc.subjectLactococcus lactisen_GB
dc.subjectGram positive bacteriaen_GB
dc.subjectStaphylococcus aureusen_GB
dc.titleBioengineered Nisin A Derivatives with Enhanced Activity against Both Gram Positive and Gram Negative Pathogensen_GB
dc.typeArticleen_GB
dc.identifier.rmisMDBY-0106-5271
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0046884
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumber10/IN.1/B3027
dc.contributor.sponsorGrantNumber06/IN.1/B98
refterms.dateFOA2018-01-12T08:13:59Z


Files in this item

Thumbnail
Name:
PLoSONe e46884.pdf
Size:
1.120Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record