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dc.contributor.authorMcAllan, Liam*
dc.contributor.authorSpeakman, John R.*
dc.contributor.authorCryan, John F.*
dc.contributor.authorNilaweera, Kanishka*
dc.date.accessioned2015-02-17T12:30:44Z
dc.date.available2015-08-01T16:00:05Z
dc.date.issued13/01/2015
dc.identifier.citationLiam McAllan, John R. Speakman, John F. Cryan and Kanishka N. Nilaweera (2015). Whey protein isolate decreases murine stomach weight and intestinal length and alters the expression of Wnt signalling-associated genes. British Journal of Nutrition, 113, pp 372-379. doi:10.1017/S0007114514004024en_GB
dc.identifier.issn1475-2662
dc.identifier.urihttp://hdl.handle.net/11019/777
dc.identifier.urihttp://dx.doi.org/10.1017/S0007114514004024
dc.identifier.urihttp://journals.cambridge.org/article_S0007114514004024
dc.descriptionK. N. N. was supported by the Teagasc Vision Programme on Obesity (RMIS5974). L. M. was supported by the Teagasc Walsh Fellowship. J. R. S.was supported by a 1000-talents professorship from the Chinese government.
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe present study examined the underlying mechanisms by which whey protein isolate (WPI) affects energy balance. C57BL/6J mice were fed a diet containing 10 % energy from fat, 70 % energy from carbohydrate (35 % energy from sucrose) and 20 % energy from casein or WPI for 15 weeks. Mice fed with WPI had reduced weight gain, cumulative energy intake and dark-phase VO2 compared with casein-fed mice (P< 0·05); however, WPI intake had no significant effects on body composition, meal size/number, water intake or RER. Plasma levels of insulin, TAG, leptin, glucose and glucagon-like peptide 1 remained unchanged. Notably, the intake of WPI reduced stomach weight and both length and weight of the small intestine (P< 0·05). WPI intake reduced the gastric expression of Wingless/int-1 5a (Wnt5a) (P< 0·01) and frizzled 4 (Fzd4) (P< 0·01), with no change in the expression of receptor tyrosine kinase-like orphan receptor 2 (Ror2) and LDL receptor-related protein 5 (Lrp5). In the ileum, WPI increased the mRNA expression of Wnt5a (P< 0·01) and caused a trend towards an increase in the expression of Fzd4 (P= 0·094), with no change in the expression of Ror2 and Lrp5. These genes were unresponsive in the duodenum. Among the nutrient-responsive genes, WPI specifically reduced ileal mRNA expression of peptide YY (P< 0·01) and fatty acid transporter protein 4 (P< 0·05), and decreased duodenal mRNA expression of the insulin receptor (P= 0·05), with a trend towards a decreased expression of Na–glucose co-transporter 1 (P= 0·07). The effects of WPI on gastrointestinal Wnt signalling may explain how this protein affects gastrointestinal structure and function and, in turn, energy intake and balance.en_GB
dc.description.sponsorshipTeagasc Walsh Fellowship Programme
dc.description.sponsorshipTeagasc Vision Programmeen_GB
dc.language.isoenen_GB
dc.publisherCambridge University Pressen_GB
dc.relation.ispartofseriesBritish Journal of Nutrition;vol 113
dc.subjectWhey proteinsen_GB
dc.subjectStomachen_GB
dc.subjectIntestineen_GB
dc.subjectEnergy Intakeen_GB
dc.titleWhey protein isolate decreases murine stomach weight and intestinal length and alters the expression of Wnt signalling-associated genesen_GB
dc.typeArticleen_GB
dc.embargo.terms13/07/2015en_GB
dc.identifier.rmisMDBY-0106-6103
refterms.dateFOA2018-01-12T08:22:09Z


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