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dc.contributor.authorO'Sullivan, Daniel*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorO'Sullivan, Orla*
dc.contributor.authorGiblin, Linda*
dc.contributor.authorMcSweeney, Paul L. H.*
dc.contributor.authorSheehan, Diarmuid (JJ)*
dc.date.accessioned2015-02-18T15:10:27Z
dc.date.available2015-08-01T16:00:06Z
dc.date.issued30/01/2015
dc.identifier.citationO'Sullivan DJ, Cotter PD, O'Sullivan O, Giblin L, McSweeney PLH, Sheehan JJ. 2015. Temporal and spatial differences in microbial composition during the manufacture of a continental-type cheese. Appl Environ Microbiol 81:2525–2533.doi:10.1128/AEM.04054-14en_GB
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/11019/780
dc.descriptionpeer-revieweden_GB
dc.description.abstractWe sought to determine if the time, within a production day, that a cheese is manufactured has an influence on the microbial community present within that cheese. To facilitate this, 16S rRNA amplicon sequencing was used to elucidate the microbial community dynamics of brine salted Continental-type cheese in cheeses produced early and late in the production day. Differences in microbial composition of the core and rind of the cheese were also investigated. Throughout ripening, it was apparent that late production day cheeses had a more diverse microbial population than their early day equivalents. Spatial variation between the cheese core and rind was also noted in that cheese rinds were found to initially have a more diverse microbial population but thereafter the opposite was the case. Interestingly, the genera Thermus, Pseudoalteromonas and Bifidobacterium, not routinely associated with a Continental-type cheese produced from pasteurised milk were detected. The significance, if any, of the presence of these genera will require further attention. Ultimately, the use of high throughput sequencing has facilitated a novel and detailed analysis of the temporal and spatial distribution of microbes in this complex cheese system and established that the period during a production cycle at which a cheese is manufactured can influence its microbial composition.en_GB
dc.description.sponsorshipThis work was funded by the Department of Agriculture, Food and the Marine under the Food Institutional Research Measure through the ‘Cheeseboard 2015’ project. Daniel J. O’Sullivan is in receipt of a Teagasc Walsh Fellowship, Grant Number: 2012205
dc.language.isoenen_GB
dc.publisherAmerican Society for Microbiologyen_GB
dc.relation.ispartofseriesApplied and Environmental Microbiology;
dc.subjectCheese microbiotaen_GB
dc.subjectNext Generation Sequencingen_GB
dc.subjectSpatialen_GB
dc.subjectTemporal distributionen_GB
dc.titleTemporal and spatial differences in microbial composition during the manufacture of a Continental-type cheeseen_GB
dc.typeArticleen_GB
dc.embargo.terms30/07/2015en_GB
dc.identifier.rmis6288
dc.identifier.doihttp://dx.doi.org/10.1128/AEM.04054-14
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorGrantNumber2012205
refterms.dateFOA2018-01-12T08:06:22Z


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