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dc.contributor.authorField, Des*
dc.contributor.authorDaly, Karen M.*
dc.contributor.authorO'Connor, Paula M.*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorHill, Colin*
dc.contributor.authorRoss, R Paul*
dc.date.accessioned2015-02-20T10:13:50Z
dc.date.available2015-09-01T16:00:05Z
dc.date.issued06/02/2015
dc.identifier.citationDes Field, Karen Daly, Paula M. O'Connor, Paul D. Cotter, Colin Hill, and R. Paul Ross. Efficacy of nisin A and nisin V semi-purified preparations alone and in combination with plant essential oils to control Listeria monocytogenes. Appl. Environ. Microbiol. AEM.00070-15; Accepted manuscript posted online 6 February 2015, doi:10.1128/AEM.00070-15en_GB
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/11019/781
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe foodborne pathogenic bacterium Listeria is known for relatively low morbidity and high mortality rates reaching up to 25-30%. Listeria is a hardy organism and its control in foods represents a significant challenge. Many naturally occurring compounds, including the bacteriocin nisin and a number of plant essential oils, have been widely studied and are reported to be effective as antimicrobial agents against spoilage and pathogenic microorganisms. The aim of this study was to investigate the ability of semi-purified preparations (spp) containing either nisin A or an enhanced bioengineered derivative nisin V, alone and in combination with low concentrations of the essential oils thymol, carvacrol and trans-cinnamaldehyde, to control L. monocytogenes in both laboratory media and model food systems. Combinations of nisin V-containing spp (25 μg/ml) with thymol (0.02%), carvacrol (0.02%) or cinnamaldehyde (0.02%) produced a significantly longer lag phase than any of the essential oil/nisin A combinations. In addition, the log reduction in cell counts achieved by the nisin V + carvacrol or nisin V + cinnamaldehyde combinations was twice that of the equivalent nisin A + essential oil treatment. Significantly, this enhanced activity was validated in model food systems against L. monocytogenes strains of food origin. We conclude that the fermentate form of nisin V in combination with carvacrol and cinnamaldehyde offers significant advantages as a novel, natural and effective means to enhance food safety by inhibiting foodborne pathogens such as L. monocytogenes.en_GB
dc.description.sponsorshipThis work was supported by the Irish Government under the National Development Plan, through Science Foundation Ireland Investigator awards to C.H. and R.P.R. (10/IN.1/B3027), and C.H., R.P.R. and P.D.C. (06/IN.1/B98).en_GB
dc.language.isoenen_GB
dc.publisherAmerican Society for Microbiologyen_GB
dc.relation.ispartofseriesApplied and Environmental Microbiology;
dc.subjectAntimicrobialen_GB
dc.subjectLantibioticen_GB
dc.subjectBacteriocinen_GB
dc.subjectPeptide engineeringen_GB
dc.subjectEssential oilsen_GB
dc.subjectThymolen_GB
dc.subjectCarvacrolen_GB
dc.subjectCinnamaldehydeen_GB
dc.subjectNisinen_GB
dc.subjectListeriaen_GB
dc.subjectBiopreservationen_GB
dc.subjectHurdle technologyen_GB
dc.subjectFood safetyen_GB
dc.titleEfficacy of nisin A and nisin V semi-purified preparations alone and in combination with plant essential oils to control Listeria monocytogenesen_GB
dc.typeArticleen_GB
dc.embargo.terms06/08/2015en_GB
dc.identifier.rmis6561
dc.identifier.doihttp://dx.doi.org/10.1128/AEM.00070-15
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumber10/IN.1/B3027
dc.contributor.sponsorGrantNumber06/IN.1/B98
refterms.dateFOA2018-01-12T08:25:20Z


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