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dc.contributor.authorGiblin, Linda
dc.contributor.authorDarimont, Christian
dc.contributor.authorLeone, Patricia
dc.contributor.authorMcNamara, Louise B.
dc.contributor.authorBlancher, Florence
dc.contributor.authorBerry, Donagh P.
dc.contributor.authorCastaneda-Gutierrez, Euridice
dc.contributor.authorLawlor, Peadar G
dc.date.accessioned2015-05-14T14:00:26Z
dc.date.available2015-05-14T14:00:26Z
dc.date.issued08/03/2015
dc.identifier.citationLinda Giblin, Christian Darimont, Patricia Leone, Louise B McNamara, Florence Blancher, Donagh Berry, Eurídice Castañeda-Gutiérrez and Peadar G Lawlor. Offspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gain. Reproductive Biology and Endocrinology, 2015, 13:16 doi:10.1186/s12958-015-0009-0en_GB
dc.identifier.issn1477-7827
dc.identifier.urihttp://hdl.handle.net/11019/793
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Excessive maternal weight gain during pregnancy impacts on offspring health. This study focused on the timing of maternal gestational weight gain, using a porcine model with mothers of normal pre-pregnancy weight. Methods Trial design ensured the trajectory of maternal gestational weight gain differed across treatments in early, mid and late gestation. Diet composition did not differ. On day 25 gestation, sows were assigned to one of five treatments: Control sows received a standard gestation diet of 2.3 kg/day (30 MJ DE/day) from early to late gestation (day 25–110 gestation). E sows received 4.6 kg food/day in early gestation (day 25–50 gestation). M sows doubled their food intake in mid gestation (day 50–80 gestation). EM sows doubled their food intake during both early and mid gestation (day 25–80 gestation). L sows consumed 3.5 kg food/day in late gestation (day 80–110 gestation). Offspring body weight and food intake levels were measured from birth to adolescence. Markers of lipid metabolism, hypertrophy and inflammation were investigated in subcutaneous adipose tissue of adolescent offspring. Results The trajectory of gestational weight gain differed across treatments. However total gestational weight gain did not differ except for EM sows who were the heaviest and fattest mothers at parturition. Offspring birth weight did not differ across treatments. Subcutaneous adipose tissue from EM offspring differed significantly from controls, with elevated mRNA levels of lipogenic (CD36, ACACB and LPL), nutrient transporters (FABP4 and GLUT4), lipolysis (HSL and ATGL), adipocyte size (MEST) and inflammation (PAI-1) indicators. The subcutaneous adipose depot from L offspring exhibited elevated levels of CD36, ACACB, LPL, GLUT4 and FABP4 mRNA transcripts compared to control offspring. Conclusions Increasing gestational weight gain in early gestation had the greatest impact on offspring postnatal growth rate. Increasing maternal food allowance in late gestation appeared to shift the offspring adipocyte focus towards accumulation of fat. Mothers who gained the most weight during gestation (EM mothers) gave birth to offspring whose subcutaneous adipose tissue, at adolescence, appeared hyperactive compared to controls. This study concluded that mothers, who gained more than the recommended weight gain in mid and late gestation, put their offspring adipose tissue at risk of dysfunction.en_GB
dc.description.sponsorshipLBMcN was in receipt of a Teagasc Walsh Fellowship. Nestle hosted LG on a sabbatical and funded the RT-PCR costen_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesReproductive Biology and Endocrinology;vol 13
dc.subjectMaternal food intakeen_GB
dc.subjectGestational weight gainen_GB
dc.subjectSubcutaneous adipose tissueen_GB
dc.titleOffspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gainen_GB
dc.typeArticleen_GB
dc.identifier.rmisPDPG-0231-5510
dc.identifier.doihttp://dx.doi.org/10.1186/s12958-015-0009-0
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
refterms.dateFOA2018-01-12T08:19:47Z


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