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dc.contributor.authorCasey, Aidan*
dc.contributor.authorFox, Edward M.*
dc.contributor.authorSchmitz-Esser, Stephan*
dc.contributor.authorCoffey, Aidan*
dc.contributor.authorMcAuliffe, Olivia*
dc.contributor.authorJordan, Kieran*
dc.date.accessioned2015-05-27T13:55:19Z
dc.date.available2015-05-27T13:55:19Z
dc.date.issued28/02/2014
dc.identifier.citationCasey A, Fox EM, Schmitz-Esser S, Coffey A, McAuliffe O and Jordan K (2014) Transcriptome analysis of Listeria monocytogenes exposed to biocide stress reveals a multi-system response involving cell wall synthesis, sugar uptake, and motility. Front. Microbiol. 5:68. doi: 10.3389/fmicb.2014.00068en_GB
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11019/802
dc.descriptionpeer-revieweden_GB
dc.description.abstractListeria monocytogenes is a virulent food-borne pathogen most often associated with the consumption of “ready-to-eat” foods. The organism is a common contaminant of food processing plants where it may persist for extended periods of time. A commonly used approach for the control of Listeria monocytogenes in the processing environment is the application of biocides such as quaternary ammonium compounds. In this study, the transcriptomic response of a persistent strain of L. monocytogenes (strain 6179) on exposure to a sub-lethal concentration of the quaternary ammonium compound benzethonium chloride (BZT) was assessed. Using RNA-Seq, gene expression levels were quantified by sequencing the transcriptome of L. monocytogenes 6179 in the presence (4 ppm) and absence of BZT, and mapping each data set to the sequenced genome of strain 6179. Hundreds of differentially expressed genes were identified, and subsequent analysis suggested that many biological processes such as peptidoglycan biosynthesis, bacterial chemotaxis and motility, and carbohydrate uptake, were involved in the response of L. monocyotogenes to the presence of BZT. The information generated in this study further contributes to our understanding of the response of bacteria to environmental stress. In addition, this study demonstrates the importance of using the bacterium's own genome as a reference when analysing RNA-Seq data.en_GB
dc.description.sponsorshipThis work was supported by the E.U.7th framework projects PROMISE (project number 265877)and FOODSEG(project number 266061).Aidan Casey was supported by the Teagasc Walsh Fellowship programme.
dc.language.isoenen_GB
dc.publisherFrontiers Media SAen_GB
dc.relation.ispartofseriesFrontiers in Microbiology;vol 5
dc.subjectListeria monocytogenesen_GB
dc.subjectRNA-seqen_GB
dc.subjectBenzethonium chlorideen_GB
dc.subjectTranscriptomeen_GB
dc.subjectGene expressionen_GB
dc.subjectBiocide stressen_GB
dc.titleTranscriptome analysis of Listeria monocytogenes exposed to biocide stress reveals a multi-system response involving cell wall synthesis, sugar uptake, and motilityen_GB
dc.typeArticleen_GB
dc.identifier.rmisMDSA-0101-6294
dc.identifier.doihttp://dx.doi.org/10.3389/fmicb.2014.00068
dc.contributor.sponsorEuropean Union
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorGrantNumber265877
dc.contributor.sponsorGrantNumber266061
refterms.dateFOA2018-01-12T08:03:36Z


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