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dc.contributor.authorDiekmann, Kerstin*
dc.contributor.authorHodkinson, Trevor R*
dc.contributor.authorFricke, Evelyn*
dc.contributor.authorBarth, Susanne*
dc.date.accessioned2015-08-14T11:50:37Z
dc.date.available2015-08-14T11:50:37Z
dc.date.issued30/07/2008
dc.identifier.citationDiekmann K, Hodkinson TR, Fricke E, Barth S (2008) An Optimized Chloroplast DNA Extraction Protocol for Grasses (Poaceae) Proves Suitable for Whole Plastid Genome Sequencing and SNP Detection. PLoS ONE 3(7): e2813. doi:10.1371/journal.pone.0002813en_GB
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11019/817
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Obtaining chloroplast genome sequences is important to increase the knowledge about the fundamental biology of plastids, to understand evolutionary and ecological processes in the evolution of plants, to develop biotechnological applications (e.g. plastid engineering) and to improve the efficiency of breeding schemes. Extraction of pure chloroplast DNA is required for efficient sequencing of chloroplast genomes. Unfortunately, most protocols for extracting chloroplast DNA were developed for eudicots and do not produce sufficiently pure yields for a shotgun sequencing approach of whole plastid genomes from the monocot grasses. Methodology/Principal Findings We have developed a simple and inexpensive method to obtain chloroplast DNA from grass species by modifying and extending protocols optimized for the use in eudicots. Many protocols for extracting chloroplast DNA require an ultracentrifugation step to efficiently separate chloroplast DNA from nuclear DNA. The developed method uses two more centrifugation steps than previously reported protocols and does not require an ultracentrifuge. Conclusions/Significance The described method delivered chloroplast DNA of very high quality from two grass species belonging to highly different taxonomic subfamilies within the grass family (Lolium perenne, Pooideae; Miscanthus×giganteus, Panicoideae). The DNA from Lolium perenne was used for whole chloroplast genome sequencing and detection of SNPs. The sequence is publicly available on EMBL/GenBank.en_GB
dc.language.isoenen_GB
dc.publisherPLoSen_GB
dc.relation.ispartofseriesPLoS ONE;vol 3(7)
dc.subjectGrassesen_GB
dc.subjectChloroplastsen_GB
dc.subjectPlant Genomicsen_GB
dc.titleAn Optimized Chloroplast DNA Extraction Protocol for Grasses (Poaceae) Proves Suitable for Whole Plastid Genome Sequencing and SNP Detectionen_GB
dc.typeArticleen_GB
dc.identifier.rmisOPBY-0302-5532
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0002813
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
refterms.dateFOA2018-01-12T08:06:02Z


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