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dc.contributor.authorO'Shea, Eileen F.*
dc.contributor.authorO'Connor, Paula M.*
dc.contributor.authorRaftis, Emma J.*
dc.contributor.authorO'Toole, Paul W.*
dc.contributor.authorSTANTON, CATHERINE*
dc.contributor.authorCotter, Paul D.*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorHill, Colin*
dc.date.accessioned2012-08-20T13:52:32Z
dc.date.available2012-08-20T13:52:32Z
dc.date.issued07/10/2011
dc.identifier.citationEileen F. O’ Shea, Paula M. O’ Connor, Emma J. Raftis et al. Production of Multiple Bacteriocins from a Single Locus by Gastrointestinal Strains of Lactobacillus salivarius. J. Bacteriol. 2011; vol. 193, no. 24: 6973-698.: DOI: 10.1128/JB.06221-11en_GB
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/11019/86
dc.descriptionpeer-revieweden_GB
dc.description.abstractBacteriocins produced by Lactobacillus salivarius isolates derived from gastrointestinal origin have previously demonstrated efficacy for in vivo protection against Listeria monocytogenes infection. In this study, comparative genomic analysis was employed to investigate the intraspecies diversity of seven L. salivarius isolates of human and porcine intestinal origin, based on the genome of the well characterised bacteriocin-producing strain L. salivarius UCC118. This revealed a highly conserved megaplasmid-encoded gene cluster in these strains involved in the regulation and secretion of two-component class IIb bacteriocins. However, considerable intraspecific variation was observed in the structural genes encoding the bacteriocin peptides. These ranged from close relatives of abp118 such as salivaricin P, which differs by 2 amino acids, to completely novel bacteriocins such as salivaricin T, which is characterized in this study. Salivaricin T inhibits closely related lactobacilli and bears little homology to previously characterized salivaricins. Interestingly, the two peptides responsible for salivaricin T activity, SalTα and SalTβ, share considerable identity with the component peptides of thermophilin 13, a bacteriocin produced by Streptococcus thermophilus. Furthermore, the salivaricin locus of strain DPC6488 also encodes an additional novel one-component class IId anti-listerial bacteriocin, salivaricin L. These findings suggest a high level of redundancy in the bacteriocins that can be produced by intestinal L. salivarius isolates using the same enzymatic production and export machinery. Such diversity may contribute to their ability to dominate and compete within the complex microbiota of the mammalian gut.en_GB
dc.description.sponsorshipFood Institutional Research Measure of the Department of Agriculture, Fisheries and Food (grant no. 04R & DC); the Alimentary Pharmabiotic Centre; Science Foundation Ireland (SFI) (grant no. 07/CE/B1368)en_GB
dc.language.isoenen_GB
dc.publisherAmerican Society for Microbiologyen_GB
dc.relation.ispartofseriesJournal of Bacteriology;vol. 193
dc.subjectL. salivariusen_GB
dc.subjectbacteriocinen_GB
dc.subjectsalivaricinen_GB
dc.subjectprobioticen_GB
dc.subjectcomparative genomic hybridizationen_GB
dc.titleProduction of multiple bacteriocins from a single locus by gastrointestinal strains of Lactobacillus salivariusen_GB
dc.typeArticleen_GB
dc.identifier.rmis5271
dc.identifier.doihttp://dx.doi.org/10.1128/​JB.06221-11
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Ireland
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumber04R & DC
dc.contributor.sponsorGrantNumber07/CE/B1368
refterms.dateFOA2018-01-12T07:24:40Z


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