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dc.contributor.authorCarberry, Ciara, A*
dc.contributor.authorKenny, David, A*
dc.contributor.authorKelly, Alan, K*
dc.contributor.authorWaters, Sinead M.*
dc.date.accessioned2015-09-25T16:09:04Z
dc.date.available2015-09-25T16:09:04Z
dc.date.issued2014-08-22
dc.identifier.citationCiara A Carberry, David A Kenny, Alan K Kelly and Sinéad M Waters. Quantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake (RFI) offered contrasting diets. Journal of Animal Science and Biotechnology. 2014, Aug 22;5(1):41en_GB
dc.identifier.urihttp://hdl.handle.net/11019/876
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Methane (CH4) emissions in cattle are an undesirable end product of rumen methanogenic fermentative activity as they are associated not only with negative environmental impacts but also with reduced host feed efficiency. The aim of this study was to quantify total and specific rumen microbial methanogenic populations in beef cattle divergently selected for residual feed intake (RFI) while offered (i) a low energy high forage (HF) diet followed by (ii) a high energy low forage (LF) diet. Ruminal fluid was collected from 14 high (H) and 14 low (L) RFI animals across both dietary periods. Quantitative real time PCR (qRT-PCR) analysis was conducted to quantify the abundance of total and specific rumen methanogenic microbes. Spearman correlation analysis was used to investigate the association between the relative abundance of methanogens and animal performance, rumen fermentation variables and diet digestibility. Results Abundance of methanogens, did not differ between RFI phenotypes. However, relative abundance of total and specific methanogen species was affected (P < 0.05) by diet type, with greater abundance observed while animals were offered the LF compared to the HF diet. Conclusions These findings suggest that differences in abundance of specific rumen methanogen species may not contribute to variation in CH4 emissions between efficient and inefficient animals, however dietary manipulation can influence the abundance of total and specific methanogen species.en_GB
dc.description.sponsorshipFunding for the development and main work of this research was provided under the National Development Plan, through the Research Stimulus Fund, administered by the Department of Agriculture, Fisheries & Food, Ireland RSF 05 224.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesJournal of Animal Science and Biotechnology;vol 5
dc.subjectBovineen_GB
dc.subjectqRT-PCRen_GB
dc.subjectResidual feed intakeen_GB
dc.subjectRumen methaongensen_GB
dc.titleQuantitative analysis of ruminal methanogenic microbial populations in beef cattle divergent in phenotypic residual feed intake (RFI) offered contrasting dietsen_GB
dc.typeArticleen_GB
dc.date.updated2015-09-17T13:56:32Z
dc.language.rfc3066en
dc.rights.holderCarberry et al.; licensee BioMed Central Ltd.
dc.identifier.doihttp://dx.doi.org/10.1186/2049-1891-5-41
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
dc.contributor.sponsorGrantNumberRSF 05 224
refterms.dateFOA2018-01-12T08:19:32Z


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