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dc.contributor.authorWalsh, Calum J.*
dc.contributor.authorGuinane, Caitriona M.*
dc.contributor.authorHill, Colin*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorO'Toole, Paul W.*
dc.contributor.authorCotter, Paul D.*
dc.date.accessioned2015-10-01T16:10:15Z
dc.date.available2015-10-01T16:10:15Z
dc.date.issued16/09/2015
dc.identifier.citationCalum J. Walsh, Caitriona M. Guinane, Colin Hill, R. Paul Ross, Paul W. O’Toole and Paul D. Cotter. In silico identification of bacteriocin gene clusters in the gastrointestinal tract, based on the Human Microbiome Project’s reference genome database. BMC Microbiology. 2015, 15(1):183en_GB
dc.identifier.urihttp://hdl.handle.net/11019/881
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground The human gut microbiota comprises approximately 100 trillion microbial cells which significantly impact many aspects of human physiology - including metabolism, nutrient absorption and immune function. Disturbances in this population have been implicated in many conditions and diseases, including obesity, type-2 diabetes and inflammatory bowel disease. This suggests that targeted manipulation or shaping of the gut microbiota, by bacteriocins and other antimicrobials, has potential as a therapeutic tool for the prevention or treatment of these conditions. With this in mind, several studies have used traditional culture-dependent approaches to successfully identify bacteriocin-producers from the mammalian gut. In silico-based approaches to identify novel gene clusters are now also being utilised to take advantage of the vast amount of data currently being generated by next generation sequencing technologies. In this study, we employed an in silico screening approach to mine potential bacteriocin clusters in genome-sequenced isolates from the gastrointestinal tract (GIT). More specifically, the bacteriocin genome-mining tool BAGEL3 was used to identify potential bacteriocin producers in the genomes of the GIT subset of the Human Microbiome Project’s reference genome database. Each of the identified gene clusters were manually annotated and potential bacteriocin-associated genes were evaluated. Results We identified 74 clusters of note from 59 unique members of the Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria and Synergistetes. The most commonly identified class of bacteriocin was the >10 kDa class, formerly known as bacteriolysins, followed by lantibiotics and sactipeptides. Conclusions Multiple bacteriocin gene clusters were identified in a dataset representative of the human gut microbiota. Interestingly, many of these were associated with species and genera which are not typically associated with bacteriocin production.en_GB
dc.description.sponsorshipCJW, CMG and PDC are supported by a SFI PI award to PDC “Obesibiotics” (11/PI/1137).en_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Microbiology;vol 15
dc.subjectGut microbiotaen_GB
dc.subjectBacteriocinsen_GB
dc.subjectHuman Microbiome Projecten_GB
dc.subjectbacteriocin gene clustersen_GB
dc.titleIn silico identification of bacteriocin gene clusters in the gastrointestinal tract, based on the Human Microbiome Project’s reference genome databaseen_GB
dc.typeArticleen_GB
dc.date.updated2015-09-17T13:56:35Z
dc.language.rfc3066en
dc.rights.holderWalsh et al.
dc.identifier.doihttp://dx.doi.org/10.1186/s12866-015-0515-4
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberSFI/11/PI/1137
refterms.dateFOA2018-01-12T08:13:37Z


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