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dc.contributor.authorHogan, Ian*
dc.contributor.authorDoherty, Michael L.*
dc.contributor.authorFagan, John*
dc.contributor.authorKennedy, Emer*
dc.contributor.authorConneely, Muireann*
dc.contributor.authorBrady, Paula*
dc.contributor.authorRyan, Clare*
dc.contributor.authorLorenz, Ingrid*
dc.date.accessioned2015-10-05T15:33:29Z
dc.date.available2015-10-05T15:33:29Z
dc.date.issued2015-08-25
dc.identifier.citationIIan Hogan, Michael Doherty, John Fagan, Emer Kennedy, Muireann Conneely, Paula Brady, Clare Ryan and Ingrid Lorenz . Comparison of rapid laboratory tests for failure of passive transfer in the bovine. Irish Veterinary Journal. 2015, 68(1):18en_GB
dc.identifier.urihttp://hdl.handle.net/11019/888
dc.identifier.urihttp://dx.doi.org/10.1186/s13620-015-0047-0
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground Failure of passive transfer of maternal immunity via colostrum can occur in the bovine, and a number of blood tests have been developed to test calves for this failure. It is not clear which test is most suitable for this purpose. The objective was to examine the most commonly used tests for failure of passive transfer and to decide which is most suitable for routine laboratory use. 126 serum samples were taken from calves of dairy cows after birth but prior to colostrum feeding, and at 48 h of age. Five different tests were compared against radial immunodiffusion which is considered the appropriate reference method. These tests were serum gamma-glutamyltransferase levels, serum protein levels, serum globulin levels, an enzyme linked immunosorbent assay and the zinc sulphate turbidity test. Results The tests examined displayed high sensitivity but widely varying specificity. Examination of the use of different cut-off points allowed some improvement in specificity at the expense of sensitivity, but the tests which had performed best at the original cut-off points still displayed the best performance. Gamma-glutamyltransferase levels as a measure of colostrum absorption returned, in this study, the best balance between sensitivity and specificity. The ELISA used in this study and serum globulin levels displayed performance similar to the gamma-glutamyltransferase levels. Serum total protein was less successful than others examined at providing both sensitivity and specificity but may, when performed via refractometer, be useful for on-farm testing. As currently performed the poor sensitivity for which the zinc sulphate turbidity test is most often criticized is evident. Modification of the cut-off point to increase specificity is less successful at balancing these parameters than the ELISA, gamma-glutamyltransferase levels, and globulin levels. Conclusions Gamma-glutamyltransferase levels, ELISA testing and circulating globulin levels performed best in detecting failure of passive transfer in serum samples, although all three had some practical considerations.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesIrish Veterinary Journal;vol 68
dc.subjectFailure of passive transferen_GB
dc.subjectCalvesen_GB
dc.subjectLaboratory testsen_GB
dc.subjectDisadvantages in practicalityen_GB
dc.titleComparison of rapid laboratory tests for failure of passive transfer in the bovineen_GB
dc.typeArticleen_GB
dc.date.updated2015-09-17T13:56:37Z
dc.language.rfc3066en
dc.rights.holderHogan et al.
refterms.dateFOA2018-01-12T08:14:29Z


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