• The Development and/or Validation of Novel Intervention Technologies to Assure Meat Food Safety

      Bolton, Declan; Byrne, Brian; Lyng, James G.; Downey, Gerard; Department of Agriculture, Food and the Marine, Ireland; Food Safety Authority of Ireland (Teagasc, 01/02/2007)
      This project was undertaken to fill some of the knowledge gaps in meat food safety from farm to fork. The data provide the scientific basis for a clean sheep policy to reduce the impact of fleece as a source of microbial contamination on ovine carcasses at the beginning of the slaughter process. At the other end of the slaughter-line, a polyurethane sponge swabbing technology was developed for ovine and bovine carcass sampling as required in 2001/471/EC and the new European Commission Hygiene Regulations. At the processing stages, studies were undertaken to determine the most effective media for the recovery and culture of Cl. perfringens cells and spores; the results were then applied to thermal inactivation studies on these bacteria. Thermal resistance data were also obtained for Bacillus cereus and a radio frequency cook for meat products was validated in terms of the destruction of Cl. perfringens and B. cereus cells and spores. Finally, an aerobiology study investigated the effectiveness of a range on measures to prevent air acting as a vector for bacterial dispersion in a meat processing plant.
    • The development and/or validation of novel intervention technologies to assure meat food safety

      Bolton, Declan; Byrne, Brian; Lyng, James G. (Teagasc, 2007-02)
      This project was undertaken to fill some of the knowledge gaps in meat food safety from farm to fork. The data provide the scientific basis for a clean sheep policy to reduce the impact of fleece as a source of microbial contamination on ovine carcasses at the beginning of the slaughter process. At the other end of the slaughter-line, a polyurethane sponge swabbing technology was developed for ovine and bovine carcass sampling as required in 2001/471/EC and the new European Commission Hygiene Regulations. At the processing stages, studies were undertaken to determine the most effective media for the recovery and culture of Cl. perfringens cells and spores; the results were then applied to thermal inactivation studies on these bacteria. Thermal resistance data were also obtained for Bacillus cereus and a radio frequency cook for meat products was validated in terms of the destruction of Cl. perfringens and B. cereus cells and spores. Finally, an aerobiology study investigated the effectiveness of a range on measures to prevent air acting as a vector for bacterial dispersion in a meat processing plant.
    • Efficacy of ultraviolet light (UV-C) and pulsed light (PL) for the microbiological decontamination of raw salmon (Salmo salar) and food contact surface materials

      Pedrós-Garrido, S.; Condón-Abanto, S.; Clemente, I.; Beltrán, J.A.; Lyng, James G.; Bolton, Declan; Brunton, Nigel; Whyte, Paul; Department of Agriculture, Food and the Marine; 13F458 (Elsevier, 2018-10-03)
      The decontamination effect of two light-based technologies on salmon, polyethylene (PE) and stainless steel (SS) was evaluated. Optimization of treatment conditions for ultraviolet light (UV-C) and pulsed light (PL) was carried out on raw salmon, obtaining inactivation levels of 0.9 and 1.3 log CFU/g respectively. The effects of treatments on several microbial groups present in salmon were then evaluated. For both technologies, Pseudomonas spp. were found to be the most resistant group of microorganisms tested. Three different strains from within this group were isolated and speciated, including a P. fluorescens strain which was selected for subsequent studies. PE and SS surfaces were inoculated with a suspension of the P. fluorescens suspended in a ‘salmon juice’ solution, and treated with UV-C and PL at different doses (mJ/cm2). PE surfaces were effectively decontaminated a low doses for both technologies, with a reduction of >4 log cycles observed. Decontamination of SS was also effective when treated with PL, although at higher doses than for PE. When SS was treated with UV-C, the maximum reduction of P. fluorescens achieved was 2 log cycles, even at the highest dose.