• Login
    View Item 
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of T-StórCommunitiesPublication DateAuthorsTitlesSubjectsFunderThis CollectionPublication DateAuthorsTitlesSubjectsFunderProfilesView

    My Account

    LoginRegister

    Information

    Deposit AgreementLicense

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    High-throughput DNA sequencing to survey bacterial histidine and tyrosine decarboxylases in raw milk cheeses

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    12866_2015_Article_596.pdf
    Size:
    1.089Mb
    Format:
    PDF
    Download
    Author
    O'Sullivan, Daniel
    Fallico, Vincenzo
    O'Sullivan, Orla cc
    McSweeney, Paul L. H.
    Sheehan, Diarmuid (JJ) cc
    Cotter, Paul D.
    Giblin, Linda
    Keyword
    high-throughput DNA sequencing
    histamine- and tyramine-producing bacteria
    Cheese
    biogenic amine production
    Date
    17/11/2015
    
    Metadata
    Show full item record
    Statistics
    Display Item Statistics
    URI
    http://hdl.handle.net/11019/909
    Citation
    Daniel J. O’Sullivan, Vincenzo Fallico, Orla O’Sullivan, Paul L. H. McSweeney, Jeremiah J. Sheehan. BMC Microbiology. 2015, 5(1),266
    Abstract
    Background: The aim of this study was to employ high-throughput DNA sequencing to assess the incidence of bacteria with biogenic amine (BA; histamine and tyramine) producing potential from among 10 different cheeses varieties. To facilitate this, a diagnostic approach using degenerate PCR primer pairs that were previously designed to amplify segments of the histidine (hdc) and tyrosine (tdc) decarboxylase gene clusters were employed. In contrast to previous studies in which the decarboxylase genes of specific isolates were studied, in this instance amplifications were performed using total metagenomic DNA extracts. Results: Amplicons were initially cloned to facilitate Sanger sequencing of individual gene fragments to ensure that a variety of hdc and tdc genes were present. Once this was established, high throughput DNA sequencing of these amplicons was performed to provide a more in-depth analysis of the histamine- and tyramine-producing bacteria present in the cheeses. High-throughput sequencing resulted in generation of a total of 1,563,764 sequencing reads and revealed that Lactobacillus curvatus, Enterococcus faecium and E. faecalis were the dominant species with tyramine producing potential, while Lb. buchneri was found to be the dominant species harbouring histaminogenic potential. Commonly used cheese starter bacteria, including Streptococcus thermophilus and Lb. delbreueckii, were also identified as having biogenic amine producing potential in the cheese studied. Molecular analysis of bacterial communities was then further complemented with HPLC quantification of histamine and tyramine in the sampled cheeses. Conclusions: In this study, high-throughput DNA sequencing successfully identified populations capable of amine production in a variety of cheeses. This approach also gave an insight into the broader hdc and tdc complement within the various cheeses. This approach can be used to detect amine producing communities not only in food matrices but also in the production environment itself.
    Funder
    Teagasc Walsh Fellowship Programme; Department of Agriculture, Food and the Marine; 2012205
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1186/s12866-015-0596-0
    Scopus Count
    Collections
    Teagasc publications in Biomed Central
    Teagasc publications in Biomed Central
    Teagasc publications in Biomed Central
    Food Biosciences

    entitlement

     
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.