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dc.contributor.authorDestefanis, M.*
dc.contributor.authorNagy, Istvan*
dc.contributor.authorRigney, Brian*
dc.contributor.authorBryan, Glenn J*
dc.contributor.authorMcLean, Karen*
dc.contributor.authorHein, Ingo*
dc.contributor.authorGriffin, Denis*
dc.contributor.authorMilbourne, Dan*
dc.date.accessioned2015-11-30T16:18:59Z
dc.date.available2015-11-30T16:18:59Z
dc.date.issued24/10/2015
dc.identifier.citationMarialaura Destefanis, Istvan Nagy, Brian Rigney, Glenn J Bryan, Karen McLean, Ingo Hein, Denis Griffin and Dan Milbourne. A disease resistance locus on potato and tomato chromosome 4 exhibits a conserved multipartite structure displaying different rates of evolution in different lineages. BMC Plant Biology. 2015 Oct 24;15(1):255. DOI:doi:10.1186/s12870-015-0645-8en_GB
dc.identifier.urihttp://hdl.handle.net/11019/913
dc.descriptionpeer-revieweden_GB
dc.description.abstractBackground In plant genomes, NB-LRR based resistance (R) genes tend to occur in clusters of variable size in a relatively small number of genomic regions. R-gene sequences mostly differentiate by accumulating point mutations and gene conversion events. Potato and tomato chromosome 4 harbours a syntenic R-gene locus (known as the R2 locus in potato) that has mainly been examined in central American/Mexican wild potato species on the basis of its contribution to resistance to late blight, caused by the oomycete pathogen Phytophthora infestans. Evidence to date indicates the occurrence of a fast evolutionary mode characterized by gene conversion events at the locus in these genotypes. Results A physical map of the R2 locus was developed for three Solanum tuberosum genotypes and used to identify the tomato syntenic sequence. Functional annotation of the locus revealed the presence of numerous resistance gene homologs (RGHs) belonging to the R2 gene family (R2GHs) organized into a total of 4 discrete physical clusters, three of which were conserved across S. tuberosum and tomato. Phylogenetic analysis showed clear orthology/paralogy relationships between S. tuberosum R2GHs but not in R2GHs cloned from Solanum wild species. This study confirmed that, in contrast to the wild species R2GHs, which have evolved through extensive sequence exchanges between paralogs, gene conversion was not a major force for differentiation in S. tuberosum R2GHs, and orthology/paralogy relationships have been maintained via a slow accumulation of point mutations in these genotypes. Conclusions S. tuberosum and Solanum lycopersicum R2GHs evolved mostly through duplication and deletion events, followed by gradual accumulation of mutations. Conversely, widespread gene conversion is the major evolutionary force that has shaped the locus in Mexican wild potato species. We conclude that different selective forces shaped the evolution of the R2 locus in these lineages and that co-evolution with a pathogen steered selection on different evolutionary paths.en_GB
dc.description.sponsorshipThis research was funded by financial contributions from the Teagasc Walsh Fellowship Scheme, The Research Stimulus Fund of the Department of Agriculture Food and Fisheries, The European Union, and Teagasc/JHI core fundingen_GB
dc.language.isoenen_GB
dc.publisherBiomed Centralen_GB
dc.relation.ispartofseriesBMC Plant Biology;vol 15
dc.subjectPhytophthora infestansen_GB
dc.subjectPotatoen_GB
dc.subjectTomatoen_GB
dc.subjectBlight resistanceen_GB
dc.subjectNB-LRR based resistance (R) genesen_GB
dc.subjectR2 gene family (R2GHs)en_GB
dc.titleA disease resistance locus on potato and tomato chromosome 4 exhibits a conserved multipartite structure displaying different rates of evolution in different lineagesen_GB
dc.typeArticleen_GB
dc.date.updated2015-10-24T06:02:09Z
dc.language.rfc3066en
dc.rights.holderDestefanis et al.
dc.identifier.doihttp://dx.doi.org/10.1186/s12870-015-0645-8
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Ireland
dc.contributor.sponsorEuropean Commission
refterms.dateFOA2018-01-12T08:24:34Z


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