• Login
    View Item 
    •   T-Stór
    • Food Programme
    • Food Biosciences
    • View Item
    •   T-Stór
    • Food Programme
    • Food Biosciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of T-StórCommunitiesPublication DateAuthorsTitlesSubjectsFunderThis CollectionPublication DateAuthorsTitlesSubjectsFunderProfilesView

    My Account

    LoginRegister

    Information

    Deposit AgreementLicense

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Impact of Environmental Factors on Bacteriocin Promoter Activity in Gut-Derived Lactobacillus salivarius

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Guinane et al AEM 2015.pdf
    Size:
    888.2Kb
    Format:
    PDF
    Download
    Author
    Guinane, Caitriona M.
    Piper, Clare
    Draper, Lorraine A.
    O'Connor, Paula M.
    Hill, Colin
    Ross, R Paul
    Cotter, Paul D.
    Keyword
    Bacteriocins
    Promoter
    Regulation
    Green Fluorescent Protein
    Date
    04/09/2015
    
    Metadata
    Show full item record
    Statistics
    Display Item Statistics
    URI
    http://hdl.handle.net/11019/917
    Citation
    Guinane CM, Piper C, Draper LA, O’Connor PM, Hill C, Ross RP, Cotter PD. 2015. Impact of environmental factors on bacteriocin promoter activity in gut derived Lactobacillus salivarius. Appl Environ Microbiol 81:7851–7859. doi:10.1128/AEM.02339-15
    Abstract
    Bacteriocin production is regarded as a desirable probiotic trait that aids in colonization and persistence in the gastrointestinal tract (GIT). Strains of Lactobacillus salivarius, a species associated with the GIT, are regarded as promising probiotic candidates and have a number of associated bacteriocins documented to date. These include multiple class IIb bacteriocins (salivaricin T, salivaricin P, and ABP-118) and the class IId bacteriocin bactofencin A, which show activity against medically important pathogens. However, the production of a bacteriocin in laboratory media does not ensure production under stressful environmental conditions, such as those encountered within the GIT. To allow this issue to be addressed, the promoter regions located upstream of the structural genes encoding the L. salivarius bacteriocins mentioned above were fused to a number of reporter proteins (green fluorescent protein [GFP], red fluorescent protein [RFP], and luciferase [Lux]). Of these, only transcriptional fusions to GFP generated signals of sufficient strength to enable the study of promoter activity in L. salivarius. While analysis of the class IIb bacteriocin promoter regions indicated relatively weak GFP expression, assessment of the promoter of the antistaphylococcal bacteriocin bactofencin A revealed a strong promoter that is most active in the absence of the antimicrobial peptide and is positively induced in the presence of mild environmental stresses, including simulated gastric fluid. Taken together, these data provide information on factors that influence bacteriocin production, which will assist in the development of strategies to optimize in vivo and in vitro production of these antimicrobials.
    Funder
    Science Foundation Ireland
    Grant Number
    SFI/11/PI/1137
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1128/AEM.02339-15
    Scopus Count
    Collections
    Food Biosciences
    Food Biosciences
    Food Biosciences
    Food Biosciences

    entitlement

     
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.