Loading...
Development of a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in their barley host and aphid vectors
Ballandras, V. McNamara, Louise Carolan, J.C Byrne, Stephen
Ballandras, V.
McNamara, Louise
Carolan, J.C
Byrne, Stephen
Date
2024-12-07
Collections
Research Projects
Organizational Units
Journal Issue
Citation
Ballandras, V., McNamara, L., Carolan, J.C. and Byrne, S. Development of a droplet digital PCR assay to detect and quantify BYDV-MAV and BYDV-PAS in their barley host and aphid vectors. Irish Journal of Agricultural and Food Research, 2024, 63(1), pp.54-65. DOI: 10.15212/ijafr-2023-0115
Abstract
Barley yellow dwarf viruses (BYDVs) belong to a complex of several species, all vectored by aphids. Due to the
abundance of Sitobion avenae and Rhopalosiphum padi, BYDV-MAV and BYDV-PAS are among the prevalent species
in Irish crops. Several BYDV detection methods, such as immunosorbent assays and PCR-based diagnostic tests,
are available and routinely used. However, there are opportunities to develop improved assays to capture viral load
information from different sample matrices. Here, we successfully developed a droplet digital PCR assay to detect
and quantify BYDV-MAV and BYDV-PAS in both aphid and barley samples. The high specificity shown by this assay
allows us to differentiate the two species from each other within a wide dynamic range. This assay will provide a
better overview of the process underlying BYDV infection and transmission from the early stage of infection to the
appearance of the symptoms.