Donovan, A.Hanrahan, James PLally, T.Boland, MauriceByrne, G.P.Duffy, P.Lonergan, P.O'Neill, D.J.2017-08-142017-08-142001-01-01Donovan, A., et al., AI For Sheep Using Frozen-thawed Semen, End of Project Reports, Teagasc, 2001.1841701521http://hdl.handle.net/11019/1401End of Project ReportInternational experience has been that cervical insemination of sheep with frozen-thawed semen usually yields unacceptably low pregnancy rates (10 to 30%). An exceptional case has been Norway where non-return rates in on-farm usage are around 60%. The objective of the work described in this report was to develop an AI procedure for Irish conditions, based initially on Norwegian protocols, using semen from individual rams. Such a procedure would greatly facilitate and enhance genetic improvement programmes for sheep. The work undertaken had two separate aspects:- (i) studies on semen, including processing and freezing methods, laboratory evaluation of semen quality post thawing and the relationship of in vitro evaluation to fertilisation rate in vivo (ii) studies on pregnancy rate following AI in relation to issues such as ram breed effects, effects of synchronisation, operator differences and the role of ewe breed inducing the timing of ovulation and various physical and physiological assessments of the cervix at AI. The main results in relation to semen studies were that, while a range of differential staining procedures could be used to objectively evaluate semen with respect to proportion of live speramatozoa and the integrity of sperm cells after thawing, these results were not useful as indicators of fertilisation capacity in vivo. The in vitro fertilisation (IVF) of sheep oocytes recovered from abattoir material gave promising results as a method for evaluating the fertilisation capacity of frozen-thawed semen. The technique requires further validation.enSheep productionArtificial insemination (AI)frozen-thawed semenTechnical Report4047