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Recent Submissions

  • ItemOpen Access
    Exploring Ultrasound, Microwave and Ultrasound–Microwave Assisted Extraction Technologies to Increase the Extraction of Bioactive Compounds and Antioxidants from Brown Macroalgae
    (MDPI AG, 2020-3-20) Garcia-Vaquero, Marco; Ummat, Viruja; Tiwari, Brijesh; Rajauria, Gaurav; Science Foundation Ireland; 16/RC/3889
    This study aims to determine the influence of (1) ultrasound-assisted extraction (UAE), (2) microwave-assisted extraction (MAE) and (3) a combination of ultrasound–microwave-assisted extraction (UMAE) on the yields of fucose-sulphated polysaccharides (FSPs), total soluble carbohydrates and antioxidants extracted from A. nodosum. Scanning electron microscopy (SEM) was used to evaluate the influence of the extraction technologies on the surface of macroalgae while principal component analysis was used to assess the influence of the extraction forces on the yields of compounds. UMAE generated higher yields of compounds compared to UAE and MAE methods separately. The maximum yields of compounds achieved using UMAE were: FSPs (3533.75 ± 55.81 mg fucose/100 g dried macroalgae (dm)), total soluble carbohydrates (10408.72 ± 229.11 mg glucose equivalents/100 g dm) and phenolic compounds (2605.89 ± 192.97 mg gallic acid equivalents/100 g dm). The antioxidant properties of the extracts showed no clear trend or extreme improvements by using UAE, MAE or UMAE. The macroalgal cells were strongly altered by the application of MAE and UMAE, as revealed by the SEM images. Further research will be needed to understand the combined effect of sono-generated and microwave-induced modifications on macroalgae that will allow us to tailor the forces of extraction to target specific molecules.
  • ItemOpen Access
    Genotypic and Phenotypic Characterization of Fecal Staphylococcus epidermidis Isolates Suggests Plasticity to Adapt to Different Human Body Sites
    (Frontiers Media SA, 2020-4-21) Garcia-Gutierrez, Enriqueta; Walsh, Calum J.; Sayavedra, Lizbeth; Diaz-Calvo, Teresa; Thapa, Dinesh; Ruas-Madiedo, Patricia; Mayer, Melinda J.; Cotter, Paul D.; Narbad, Arjan; Biotechnology and Biological Sciences Research Council; Science Foundation Ireland; BB/R012490/1; SFI/11/PI/1137); SFI/12/RC/2273
    Staphylococcus epidermidis is a commensal species that has been increasingly identified as a nosocomial agent. Despite the interest, little is known about the ability of S. epidermidis isolates to adapt to different ecological niches through comparisons at genotype or phenotype levels. One niche where S. epidermidis has been reported is the human gut. Here, we present three S. epidermidis strains isolated from feces and show that they are not phylogenetically distinct from S. epidermidis isolated from other human body sites. Both gut and skin strains harbored multiple genes associated with biofilm formation and showed similar levels of biofilm formation on abiotic surfaces. High-throughput physiological tests using the BIOLOG technology showed no major metabolic differences between isolates from stool, skin, or cheese, while an isolate from bovine mastitis showed more phenotypic variation. Gut and skin isolates showed the ability to metabolize glycine-conjugated bile acids and to grow in the presence of bile, but the gut isolates exhibited faster anaerobic growth compared to isolates of skin origin.
  • ItemOpen Access
    Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk
    (Springer Science and Business Media LLC, 2020-3-13) Garcia-Gutierrez, Enriqueta; O’Connor, Paula M.; Colquhoun, Ian J.; Vior, Natalia M.; Rodríguez, Juan Miguel; Mayer, Melinda J.; Cotter, Paul D.; Narbad, Arjan; BBSRC Institute; Science Foundation Ireland; BB/R012490/1; BBS/E/F/000PR10356; SFI/11/PI/1137; SFI/12/RC/2273
    Bacteriocins are antimicrobial peptides produced by bacteria, and their production is regarded as a desirable probiotic trait. We found that Lactobacillus gasser LM19, a strain isolated from human milk, produces several bacteriocins, including a novel bacteriocin, gassericin M. These bacteriocins were purified from culture and synthesised to investigate their activity and potential synergy. L. gasseri LM19 was tested in a complex environment mimicking human colon conditions; it not only survived, but expressed the seven bacteriocin genes and produced short-chain fatty acids. Metagenomic analysis of these in vitro colon cultures showed that co-inoculation L. gasseri LM19 with Clostridium perfringens gave 16S ribosomal RNA metagenomic profiles with more similarity to controls than to vessels inoculated with C. perfringens alone. These results indicate the L. gasseri LM19 could be an interesting candidate for maintaining homeostasis in the gut environment.
  • ItemOpen Access
    Shotgun sequencing of the vaginal microbiome reveals both a species and functional potential signature of preterm birth
    (Springer Science and Business Media LLC, 2020-11-12) Feehily, Conor; Crosby, David; Walsh, Calum J.; Lawton, Elaine M.; Higgins, Shane; McAuliffe, Fionnuala M.; Cotter, Paul D.; Science Foundation Ireland; SFI/12/RC/2273; 16/SP/3827
    An association between the vaginal microbiota and preterm birth (PTB) has been reported in several research studies. Population shifts from high proportions of lactobacilli to mixed species communities, as seen with bacterial vaginosis, have been linked to a twofold increased risk of PTB. Despite the increasing number of studies using next-generation sequencing technologies, primarily involving 16S rRNA-based approaches, to investigate the vaginal microbiota during pregnancy, no distinct microbial signature has been associated with PTB. Shotgun metagenomic sequencing offers a powerful tool to reveal community structures and their gene functions at a far greater resolution than amplicon sequencing. In this study, we employ shotgun metagenomic sequencing to compare the vaginal microbiota of women at high risk of preterm birth (n = 35) vs. a low-risk control group (n = 14). Although microbial diversity and richness did not differ between groups, there were significant differences in terms of individual species. In particular, Lactobacillus crispatus was associated with samples from a full-term pregnancy, whereas one community state-type was associated with samples from preterm pregnancies. Furthermore, by predicting gene functions, the functional potential of the preterm microbiota was different from that of full-term equivalent. Taken together, we observed a discrete structural and functional difference in the microbial composition of the vagina in women who deliver preterm. Importance: with an estimated 15 million cases annually, spontaneous preterm birth (PTB) is the leading cause of death in infants under the age of five years. The ability to accurately identify pregnancies at risk of spontaneous PTB is therefore of utmost importance. However, no single cause is attributable. Microbial infection is a known risk factor, yet the role of vaginal microbes is poorly understood. Using high-resolution DNA-sequencing techniques, we investigate the microbial communities present in the vaginal tracts of women deemed high risk for PTB. We confirm that Lactobacillus crispatus is strongly linked to full-term pregnancies, whereas other microbial communities associate with PTB. Importantly, we show that the specific functions of the microbes present in PTB samples differs from FTB samples, highlighting the power of our sequencing approach. This information enables us to begin understanding the specific microbial traits that may be influencing PTB, beyond the presence or absence of microbial taxa.
  • PublicationOpen Access
    TResearch Volume 6: Number 4. Winter 2011
    (Teagasc, 2011-12-21)
    Page 4 News: New Teagasc sheep programme International collaboration on probiotic research UCD and Teagasc sign partnership agreement Page 8 Crops, Environment and Land Use: EUCARPIA 2011 Page 10 Crops, Environment and Land Use: Conserving farmland biodiversity Page 12 Crops, Environment and Land Use: Nutrient loads from agri-catchments: environmental risk or economic write-off? Page 14 Food: Package your way to new markets Page 16 Food: The tender touch Page 18 Food: Protein aggregation in dairy food structures Page 20 Animal production and grassland: Safe handling of cattle on farms Page 22 Animal production and grassland: The impact of mastitis on farm profitability Page 24 Animal production and grassland: Integrating basic and applied research on mastitis Page 26 Animal production and grassland: New dairy farms Page 28 Animal production and grassland: Can beef from dairy bulls be profitable? Page 30 Rural Economy and Development: Food choices – what's my motivation? Page 32 Events: Teagasc science events 2012

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