β-lactoglobulin as a molecular carrier of linoleate: characterisation and effects on intestinal epithelial cells in vitro

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dc.contributor.authorLe Maux, Solene*
dc.contributor.authorGiblin, Linda*
dc.contributor.authorCroguennec, Thomas*
dc.contributor.authorBouhallab, Said*
dc.contributor.authorBrodkorb, Andre*
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
dc.contributor.sponsorIrish Research Council for Science, Engineering and Technology
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorGrantNumber08/RD/TMFRC/650
dc.date.accessioned2014-01-16T12:49:43Z
dc.date.available2014-01-16T12:49:43Z
dc.date.issued27/08/2012
dc.descriptionS. Le Maux is currently supported by a Teagasc Walsh Fellowship and the Department of Agriculture, Fisheries and Food (FIRM project 08/RD/TMFRC/650). We also acknowledge funding from IRCSET-Ulysses Travel Grant.
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe dairy protein β-lactoglobulin (βlg) is known to bind hydrophobic ligands such as fatty acids. In the present work, we investigated the biological activity in vitro of linoleate once complexed to bovine βlg. Binding of linoleate (C18:2) to bovine βlg was achieved by heating at 60 °C for 30 min at pH 7.4, resulting in a linoleate/βlg molar binding stoichiometry of 1.1, 2.1, and 3.4. Two types of binding sites were determined by ITC titrations. Binding of linoleate induced the formation of covalent dimers and trimers of βlg. The LD50 on Caco-2 cells after 24 h was 58 μM linoleate. However, cell viability was unaffected when 200 μM linoleate was presented to the Caco-2 cells as part of the βlg complex. The Caco-2 cells did not increase mRNA transcript levels of long chain fatty acid transport genes, FATP4 and FABPpm, or increase levels of the cAMP signal, in response to the presence of 50 μM linoleate alone or as part of the βlg complex. Therefore, it is proposed that βlg can act as a molecular carrier and alter the bioaccessibility of linoleate/linoleic acid.en_GB
dc.identifier.citationLe Maux, S., Giblin, L., Croguennec, T., Bouhallab, S., & Brodkorb, A. (2012). β-lactoglobulin as a molecular carrier of linoleate: characterisation and effects on intestinal epithelial cells in vitro. Journal of Agricultural and Food Chemistry, 60(37), 9476–9483. doi: 10.1021/jf3028396en_GB
dc.identifier.doihttp://dx.doi.org/10.1021/jf3028396
dc.identifier.issn0021-8561
dc.identifier.rmisMDDT-0103-5947
dc.identifier.urihttp://hdl.handle.net/11019/506
dc.language.isoenen_GB
dc.publisherAmerican Chemical Societyen_GB
dc.relation.ispartofseriesJournal of Agricultural and Food Chemistry;vol 60
dc.subjectβ-lactoglobulinen_GB
dc.subjectSodium Linoleate/Linoleic Aciden_GB
dc.subjectStoichiometryen_GB
dc.subjectCaco-2en_GB
dc.subjectCytotoxicityen_GB
dc.titleβ-lactoglobulin as a molecular carrier of linoleate: characterisation and effects on intestinal epithelial cells in vitroen_GB
dc.typeArticleen_GB
dspace.entity.typePublication
refterms.dateFOA2018-01-12T07:55:47Z
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